With patching it really is "practice makes perfect". Yesterday I spent *hours* before I finally managed to get one. Today I was much much quicker. Softly, softly....that's the key. Being very careful about how much pressure you apply down the pipette. And it seems I can't talk and successfully patch at the same time. So from now on I have to cut out the running commentary for K's benefit.
So the neuron is nicely filled with dye now, and K is getting some images of lovely spiny dendrites.
Time for lunch.
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